 |
 |
醫(yī)學論文范文:益骨口服液對去勢大鼠骨密度與骨生物力學的影響
【摘要】 [目的]探討益骨口服液對實驗去勢大鼠骨密度和骨生物力學變化的影響。[方法]將大鼠隨機分為A、B、C、D、E五組，A組(10只)為正常對照組(假手術); B組(10只)為模型組(造模后給予蒸餾水亦即空白組);C組(10只)為陽性對照組(切除雙側卵巢并予以倍美力治療);D組(10只)為高濃度組(造模后給予含生藥1.5g/ml益骨口服液治療);E組(10只)為低濃度組(造模后給予含生藥0.5g/ml益骨口服液治療)。灌胃12周后，活殺各組動物，以雙能 X線測定骨密度及三點彎曲試驗測生物力學指標。[結果]治療組骨密度明顯高于模型組(P<0.05)，骨生物力學性能較模型組得到明顯改善(P<0.05)。結論：益骨湯能促進骨形成，能提高骨強度及生物力學性能。
【關鍵詞】 益骨口服液;骨密度;骨生物力學;去勢大鼠
Effect of YiGu Oral Solution on Ovariectomized Rats’ Bone Density and Biomechanics Yao Xinmiao，Xu Luji，Leng Tao et al Third Hospital Affiliated to Zhejiang Chinese Medical University，Hangzhou(310009)
Abstract： [Objective] To observe the effect of YiGu Oral Solution on bone structure and bone biomechanics.[Methods]Rats were randomly divided into A，B，C，D，E five groups，A group(10)for the normal control group(sham operation);B group(10)as a model group(distilled water model that is，after the blank group);C group(10)for the positive control group(resection of bilateral ovarian and western medicine treatment Premarin);D group(10)for the high concentration group(model after treatment with 1.5g/mlYiGu Oral Solution Pharmacognosy );E group(10)for the low concentration group(model after treatment with 0.5g/mlYiGu Oral Solution pharmacognostic).Twelve weeks after oral administration of live animals in each group to kill in order to dualenergy Xray density of bone mineral density determination.[Results]BMD was significantly higher than the model group(P<0.05)，than the mechanical properties of bone biology has marked improvement in the model group(P<0.05).[Conclusion] YiGu Oral Solution can promote bone formation and improve the mechanical properties of bone biology醫(yī).學全.在.線m.payment-defi.com.
Key words：YiGu Oral Solution;bone mineral density;bone biomechanics;ovariectomized rats
Fund project： Item supported by Zhejiang Provincial Scitech Bureau(No.2007C33019) 絕經后骨質疏松癥是中老年婦女常見的骨代謝性疾病[12]。通過建立去勢大鼠骨質疏松癥的動物模型，探索益骨口服液防治骨質疏松癥的作用機制。補腎健脾是中醫(yī)治療此骨質疏松癥的方法之一，本實驗將以補腎健脾胃為主的中藥復方益骨口服液應用于去勢大鼠的骨質疏松模型，觀察其對大鼠骨密度及骨生物力學的影響。
1 材料與方法
1.1 實驗藥物 益骨口服液水提液：①按照益骨口服液的提取與成型工藝，由浙江中醫(yī)藥大學實驗制劑中心加工。②倍美力水溶液：美國惠氏艾爾斯特制藥公司艾爾斯特藥廠制造，生產批號0502090，國藥準字(2001)J51(2)號。水溶液濃度為9.4ug/ml。③氯氨酮：上海第一生化藥業(yè)有限公司生產，批號滬衛(wèi)藥準字 (1995)第 001064號。
