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本實(shí)驗(yàn)通過(guò)MTT法檢測(cè)，得到粉防已堿對(duì)HeLa細(xì)胞具有抑制作用，且隨著濃度和時(shí)間的增加，抑制率逐漸增高，具有時(shí)間、濃度依賴性。為了確定粉防己堿引起的HeLa細(xì)胞增殖抑制作用是否通過(guò)凋亡途徑實(shí)現(xiàn)，本實(shí)驗(yàn)同時(shí)也利用AnnexinV/PI雙染法，經(jīng)流式細(xì)胞儀和激光共聚焦顯微鏡檢測(cè)細(xì)胞的凋亡情況，結(jié)果均證實(shí)粉防己堿對(duì)宮頸癌HeLa細(xì)胞具有促進(jìn)凋亡的作用。細(xì)胞凋亡(apoptosis)，又稱程序性細(xì)胞死亡(programmed cell death, PCD)，是指在一定生理和病理情況下機(jī)體為維持內(nèi)環(huán)境穩(wěn)定,通過(guò)基因調(diào)控而使細(xì)胞主動(dòng)有序的消亡過(guò)程,是多細(xì)胞生物的一種生理性細(xì)胞死亡。凋亡不僅對(duì)胚胎發(fā)生、器官發(fā)育及保護(hù)機(jī)體穩(wěn)態(tài)等至關(guān)重要，而且在控制細(xì)胞增殖、調(diào)節(jié)腫瘤的發(fā)生和生長(zhǎng)中起著重要的作用。凋亡負(fù)調(diào)控腫瘤的發(fā)生發(fā)展，通過(guò)凋亡機(jī)體可及時(shí)清除過(guò)多受損但來(lái)不及修復(fù)的或生理上不需要的細(xì)胞。細(xì)胞的凋亡受阻從而導(dǎo)致細(xì)胞群體凋亡和增殖的平衡受到破壞，異常細(xì)胞增殖速度超過(guò)凋亡速度，從而形成腫瘤[19]。本實(shí)驗(yàn)結(jié)果證明粉防己堿對(duì)宮頸癌HeLa細(xì)胞具有一定的促凋亡作用，有作為宮頸癌藥物治療的可能性。
在今后的實(shí)驗(yàn)中，我們將進(jìn)一步檢測(cè)粉防己堿對(duì)宮頸癌HeLa細(xì)胞是否具有周期阻滯作用，并檢測(cè)與細(xì)胞周期阻滯和凋亡相關(guān)的蛋白，更加深入的了解粉防己堿對(duì)HeLa細(xì)胞的增殖抑制的分子機(jī)制。
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